Daily Quantification of Myoglobin Forms on Beef Longissimus Lumborum Steaks Over 7 Days of Display by Near-infrared Diffuse Reflectance Spectroscopy

Near-infrared diffuse reflectance spectroscopy (NIR-DRS) was utilized to develop an algorithm using approximately 18 wavelengths spanning 480 650 nm determine oxymyoglobin (OxyMb), deoxymyoglobin (DeoxyMb), and metmyoglobin (MetMb) contents on beef longissimus lumborum muscles. Daily changes in subsurface myoglobin redox forms were evaluated for 7 d NIR-DRS compared with the surface color as assessed by a HunterLab MiniScan spectrophotometer reference modality. Both measurements revealed that MetMb increased steadily over duration of display, showing high correlation (R2 = 0.91) between 2 methods. Comparatively, whereas OxyMb have decreased period indicated much later onset apparent decrease OxyMb, resulting moderate 0.64) No found methods regarding DeoxyMb display. The newly developed has potential alternative method assessment postrigor skeletal muscle.